Nicotinamide Adenine Dinucleotide-specific Glyceraldehyde 3-Phosphate Dehydrogenase from Pisum sativum
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چکیده
منابع مشابه
Nicotinamide adenine dinucleotide-specific glyceraldehyde 3-phosphate dehydrogenase from Pisum sativum. Effect of nicotinamide adenine dinucleotide and related compounds on the enzyme-catalyzed arsenolysis of 1,3-diphosphoglyceric acid.
NADf-specific glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) from pea seeds is shown to catalyze the arsenolysis of 1 ,3-diphosphoglyceric acid. The reaction shows an absolute requirement for NAD+ or analogs which will replace NAD+ in the enzyme-catalyzed oxidative phosphorylation of glyceraldehyde 3-phosphate. NADH, glyceraldehyde j-phosphate, or NADf analogs which are inhibitory or in...
متن کاملCovalent binding of 3-pyridinealdehyde nicotinamide adenine dinucleotide and substrate to glyceraldehyde 3-phosphate dehydrogenase.
Glyceraldehyde 3-phosphate dehydrogenase (D-glyceraldehyde-3-phoshate:nicotinamide adenine dinucleotide oxidoreductase (phosphorylating), EC 1.2.1.12) forms a complex with 3-pyridinealdehyde-NAD which survives precipitation with 7% perchloric acid. The molar ratio bound 3-pyridinealdehyde-NAD to the enzyme is 2.5 to 2.9. Lactate, malate, and alcohol dehydrogenases do not form acid-precipitable ...
متن کاملA role for nicotinamide adenine dinucleotide glycohydrolase in the control of glyceraldehyde-3-phosphate dehydrogenase activity.
Tumor nicotinamide adenine dinudeotide (NAD) glycohydrolase (EC.3.2.2.5) was purified 500-fold, and this preparation was used to study the influence of NAD glycohydrolase on the NAD + content and activity of crystalline glyceraldehyde-3phosphate dehydrogenase. The pseudomonomolecular velocity constant, k, for the hydrolysis of free NAD + was 5-fold that of the constant for the enzyme-bound NAD ...
متن کاملGlyceraldehyde-3-phosphate dehydrogenase-catalyzed chain oxidation of reduced nicotinamide adenine dinucleotide by perhydroxyl radicals.
The chain oxidation of glyceraldehyde-3-phosphate dehydrogenase.NADH by perhydroxyl radicals and propagated by molecular oxygen was studied by the xanthine-xanthine oxidase system, 60Co gamma-ray, and pulse radiolysis. The chain length, amount of NADH oxidized per HO2 generated, increases with increasing acidity of the medium and reaches a value of 73 at pH 5.0. The rate constant for the oxidat...
متن کاملGlyceraldehyde - 3 - Phosphate Dehydrogenase - catalyzed Chain Oxidation of Reduced Nicotinamide Adenine Dinucleotide
The chain oxidation of glyceraldehyde-3-phosphate dehydrogenase *NADH by perhydroxyl radicals and propagated by molecular oxygen was studied by the xanthine-xanthine oxidase system, ‘OCo y-ray, and pulse radiolysis. The chain length, amount of NADH oxidized per HOz generated, increases with increasing acidity of the medium and reaches a value of 73 at pH 5.0. The rate constant for the oxidati...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43106-2